The objective of this study is to examine the effect of organizational mechanisms like leadership style, knowledge management and learning culture on ambidexterity of employees in IT sector of Pakistan. Relationship between transformational leadership and exploration and exploitation is developed, with the help of literature. Qualitative method is used to collect data through questionnaires. Data from 174 employees was processed and analyzed in SPSS. Multiple regression analysis provided us with the result that transformational leadership positively effects exploration and exploitation. This suggests that mutual contribution of leaders and employees enhance ambidextrous abilities of employees and which proves to become a competitive advantage. Mediation of knowledge sharing and learning culture propose that exchange of knowledge can draw beneficial results through exploiting existing competencies and practice of learning behavior allows exploration of new opportunities. Our findings guide leaders to support and promote ambidextrous abilities in their employees and introduce the culture of knowledge sharing and management. Supervisor:- Ms. Mariam Mohsin