This study explores the role of public transport system in female mobility in Pakistan. Based on a micro-study, using a mix method approach, this research assesses the impact of the Metro Bus System (MBS) on female mobility in the twin cities of Islamabad-Rawalpindi. Primary data is collected from three groups of female passengers – working women, students, and casual travelers – to evaluate whether the MBS has brought an improvement in the service quality of public transportation for them. The findings of this study suggest that the metro bus service in Islamabad- Rawalpindi has improved the service quality of public transportation for female passengers leading to their ease in mobility. The analysis of service quality dimensions, such as reliability, tangibility and affordability, safety and assurance shows that the MBS has effectively addressed the concerns of female population regarding public transportation environment. It has significantly improved female mobility in the twin cities by providing a respectful and hassle-free transportation. Despite some limitations such as difficulty in accessibility of metro bus stations, limited inter-connectivity, and congestion problems and unavailability of seats during peak hours, it can be concluded that the MBS has effectively addressed issues such as uncertainty and insecurity for female passengers to a greater extent. This research suggests extension of metro bus network and feeder buses to improve accessibility and interconnectivity, and running female exclusive buses during peak hours considering congestion and safety issues. Supervisor:- Dr. Faheem Jehangir Khan